Fig. 4: Binding pose and epitope of C4 and E3. | Nature Communications

Fig. 4: Binding pose and epitope of C4 and E3.

From: An original potentiating mechanism revealed by the cryo-EM structures of the human α7 nicotinic receptor in complex with nanobodies

Fig. 4

a Overview of the binding site of C4 on α7 exemplified by the C4-Apo structure. One C4 molecule (purple surface and cartoon) contacts two α7 subunits depicted in light (principal n subunit) and dark (complementary n+1 subunit) surface and cartoons. b Details of the binding site as seen from the solvent (upper panel) or the vestibule (lower panel) of α7 with color code as in (a). key residues are labeled, and their side chains displayed in stick balls. c Same as in (a) but with the E3-Apo structure, E3 is colored in green. d Same as in (b). but with the E3-Apo structure, E3 is colored in green. e Overview of the loops involved in binding exemplified by the C4-Apo structure represented in cartoons. f Interaction of E3 with Asn23 glycan. Close view of the Glycan (pink sticks) built on α7-Asn23 (pink sticks) from the pre-β1 of α7 (gray) and of the CDR2 of C4 (left, purple) or E3 (right, green). Ala57 from C4 and its homologous Arg56 from E3 are depicted in sticks.

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