Fig. 5: NLRP6 promotes glioma tumorigenesis.

a Cell proliferation assay of NLRP6-overexpressing LN229 cells compared with empty vector (EV)-transfected cells. b Cell proliferation assay of NLRP6 knockout (KO) LN229 cells compared with control cells (Ctrl). c Colony formation assay of NLRP6-overexpressing LN229 cells compared with EV. d Colony formation assay of NLRP6 KO LN229 cells compared with Ctrl. e Cell wound healing assay of NLRP6-overexpressing LN229 cells compared with EV. f Cell wound healing assay of NLRP6 KO LN229 cells compared with Ctrl. g Cell proliferation assay of Ctrl, PIK3R1 KO, or PTEN KO LN229 cells transfected with EV or NLRP6 plasmid. h In vivo bioluminescence imaging of nude mice with intracranially implanted Ctrl, PIK3R1 KO, or PTEN KO LN229 cells transfected with EV or NLRP6 plasmid. Representative bioluminescence images (left) and quantitative analysis of relative bioluminescence are shown (right). i Immunoblot analysis of NLRP6, p85α, and PTEN protein expression in GBM tissues. j Immunohistochemistry (IHC) analysis of NLRP6, p85α, and PTEN in GBM tissue paraffin sections. Scale bar = 50 μm. k Correlation between NLRP6 and p85α IHC scores. l Correlation between NLRP6 and PTEN IHC scores. m Correlation between p85α and PTEN IHC scores. p1 or p2 was the p-value for comparing NLRP6-KO-1 or NLRP6-KO-2 with Ctrl. In a-g, all error bars, mean values ± SEM, p-values were determined by unpaired two-tailed Student’s t test of n = 3 independent biological experiments. In h, all error bars, mean values ± SD, p-values were determined by unpaired two-tailed Student’s t test (n = 6 mice per group). For i, data shown are representative of three independent experiments with similar results. For k–m, p-values are determined by two-sided Pearson’s correlation test. Source data are provided as a Source Data file.