Fig. 2: ENDOG promotes lipid synthesis via the AKT-ACLY axis. | Nature Communications

Fig. 2: ENDOG promotes lipid synthesis via the AKT-ACLY axis.

From: Cytoplasmic Endonuclease G promotes nonalcoholic fatty liver disease via mTORC2-AKT-ACLY and endoplasmic reticulum stress

Fig. 2

a, b Western blot analyses of activation of AKT-ACLY in ENDOG overexpressing and knockout HepG2. pK-Myc and ENDOG overexpressing plasmids were transfected for 48 h. c Measurement of acetyl-CoA in ENDOG overexpressing / knockout HepG2. n = 6 biologically independent samples each group. d Measurement of acetyl-CoA in Endog+/- and Endog-/- mice livers. n = 6 mice in Endog+/- group and 7 mice in Endog-/- group. e–g Representative images of Nile red, the quantitative results of lipid area, and measurement of triglycerides in wild-type and ENDOG knockout HepG2 after the supplementation of 10 mM citrate for 24 h. n = 5 biologically independent samples for Nile red staining and 4 for triglyceride measurement. h–j Representative images of Nile red, the quantitative results of lipid area, and measurement of triglycerides in wild-type and ENDOG knockout HepG2 after supplementing 50 μM acetyl-CoA for 24 h. n = 6 biologically independent samples for Nile red staining and 4 for triglyceride measurement. k Western blot analyses of AKT-ACLY signaling in ENDOG overexpressing HepG2 following 50 or 100 μM LY294002 treatment for 24 h. pK-Myc and ENDOG overexpressed plasmids were transfected for 48 h. l–n Representative images of Nile red, the quantitative results of lipid area and measurement of ENDOG overexpressing HepG2 following the treatment of 200 μM oleic acid and 50 μM LY294002 for 24 h. n = 5 biologically independent samples for Nile red staining and 4 for triglyceride measurement. o Western blot analyses of AKT-ACLY signaling in wild-type and ENDOG knockout HepG2 after transfection with myr-AKT plasmid for 48 h. p–r Representative images of Nile red, the quantitative results of lipid area, and measurement of triglycerides in the indicated groups. Cells were transfected with plasmids for 24 h and then treated with 200 μM oleic acid for another 24 h. n = 5 biologically independent samples for Nile red staining and 4-6 for triglyceride measurement. Statistical significance was determined by unpaired Student’s t-test (two-tailed) in (c, d, f, g, i, j, m, n, q, r); error bars are mean ± SD. Source data and exact P values are provided in a Source data file. *P < 0.05; **P < 0.01; ***P < 0.001; n.s.: no significance.

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