Fig. 3: Diverse geographic origins of admixed Dunedin possums lead to high genetic diversity.

a Standardised multilocus heterozygosity (sMLH, upper left) of Australian and Dunedin possums, obtained from RNA-sequencing variant calls (biologically independent animals, n = 10, 1, 7, 25, 1 and 45, left to right, respectively). Boxplot minima and maxima (large black dots) indicate points beyond whiskers. Whiskers are 1.5*interquartile range above and below the 75th percentile and 25th percentile (upper and lower whisker respectively), which form the bounds of the box, with the centre of the box representing the median. Clades were obtained from the maximum likelihood phylogenetic reconstruction of mitogenomes, utilising reference samples from New South Wales (NSW, light green), Kangaroo Island (KAN, beige), and Tasmania (TAS, dark green). Haplotypes from NSW and TAS, but not KAN, were present in Dunedin samples, as well as two likely mainland Australian haplotypes, identified by homology to D-loop sequence⁴⁸ (red and blue). b Nuclear admixture analysis for Dunedin samples relative to reference samples from TAS (dark green) and NSW/KAN (light green), as well as another likely mainland source (blue). Results for Sandy, the reference genome individual, are indicated (star). Source data are provided as a Source Data file.