Fig. 3: Validation of the Lr47 candidate gene using EMS mutants.
From: Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides
a Susceptible EMS mutants used to isolate the Lr47 candidate gene. Infection types for Kern Lr47 (positive control), ten independent EMS mutants, and Kern (negative control) inoculated with Pt race THDB. R resistant, S susceptible. b Gene structure of the Lr47 candidate gene. The positions of the EMS-derived loss-of-function mutations are indicated by blue arrows. The mutant line m1541 carries two mutations. Gray boxes indicate untranslated regions, blue boxes represent coding exons, and dotted lines represent introns. c Protein structure of Lr47, with the predicted amino acid changes caused by the EMS mutations indicated. Mutation names in red indicate premature stop codons and in black indicate amino acid substitutions. CC domain, NB domain, and LRR motifs are shaded with yellow, green, and blue, respectively. Source data are provided as a Source data file.
