Fig. 1: Centrosome characterisation in HGSOC tissue samples.
From: Molecular landscape and functional characterization of centrosome amplification in ovarian cancer
a Basic workflow of centrosome characterisation in clinical tissue samples (also see Supplementary Methods). Example confocal immunofluorescent images (max. intensity projections) of a fallopian tube and HGSOC tissue are shown in (b) and (c), respectively (left panel). Nuclei are shown in blue, centrosomes are shown in green. Individually detected centrosomes following automated image analysis are highlighted in rainbow colours (right panel; nuclei shown in grey). Scale bars = 50 µm. d, e show sample type comparison of centrosome amplification (CA) scores and centrosome size, respectively. Sample types are indicated by different colours: Normal (other), pink, n = 2; Normal (FT), orange, n = 24; HGSOC (OV04), light blue, n = 93; HGSOC (BriTROC), dark blue, n = 194; Liver, purple, n = 5. Statistics shown is a Kruskal–Wallis one-way analysis of variance test. Boxplots show 25th, 50th and 75th centiles; whiskers indicate 75th centile plus 1.5 × inter-quartile range and 25th centile less 1.5 × inter-quartile range. Notches on boxes extend 1.58 × inter-quartile range/sqrt(n) approximating to the 95% confidence interval for comparing medians. Dashed horizontal line indicates CA cutoff threshold of ~1.83. Source data are provided as a Source Data file.
