Fig. 3: Centrosome amplification shows inter- and intra-tissue heterogeneity.

Spatial heatmap of centrosome amplification in a normal fallopian tube (a) and HGSOC tumour tissue (b). Additional examples are shown in Supplementary Fig. 1. CA scores are indicated by a colour gradient (purple = low, yellow = high) for each imaging field. Points represent individual nuclei detected during image analyses and were plotted in relation to their physical position (µm) on the microscope slide (x and y axis). Note that CMYK printing may obscure differences on the CA score colour scale. c Comparison of CA scores across tissues collected from individual patients in a total of 36 cases. Red stars indicate CA score medians for patient with multiple tissue samples. d Comparison of CA scores across different tissue sites (n = 287 individual samples; BriTROC, n = 194; OV04, n = 93). e Comparison of CA tissue heterogeneity across different tissue sites (n = 287 individual samples; BriTROC, n = 194; OV04, n = 93). Statistics shown for (d) and (e) is a Kruskal–Wallis one-way analysis of variance test. Tissue sites are indicated by different shapes. Cohorts are indicated by teal (OV04) and light blue (BriTROC) colours. Dashed horizontal lines indicate the CA score cutoff of ~1.83. Boxplots show 25th, 50th and 75th centiles; whiskers indicate 75th centile plus 1.5 × inter-quartile range and 25th centile less 1.5 × inter-quartile range. Notches on boxes extend 1.58 × inter-quartile range/sqrt(n) approximating to the 95% confidence interval for comparing medians. Source data are provided as a Source Data file.