Fig. 6: Centrosome amplification is associated with increased micronuclei frequencies and can be induced by low oxygen growth conditions.

Confocal immunofluorescent staining images (zoomed in from 40× max. intensity projection images) for (a) CA-low and (b) CA-high cell lines as highlighted in Fig. 5b. Cells were stained for Pericentrin (yellow), pHH3 (phospho-histone H3; mitotic cells; red), and DNA (Hoechst; blue). Scale bar = 50 µm. c Cell line characterisation correlation plot showing Spearman’s rank correlations (two-sided) of analysed immunofluorescent staining results. P-values: *** p < 0.001; ** p < 0.01; * p < 0.05;. p < 0.1. Error bands show 95% confidence intervals. d–e Cell line subtype comparison of centrosome amplification and micronuclei frequencies, respectively (n = 73 individual cell lines; HGSOC, n = 57; other, n = 16). Histological subtypes are indicated by different colours. Note that the three “normal” cell lines were grouped together with other subtypes, as these cell lines are transformed and show some cancer characteristics. f–g Centrosome amplification and micronuclei frequencies, respectively, in cell lines grown at 21% vs. 5% oxygen (n = 73 individual cell lines; 21% oxygen, n = 48; 5% oxygen, n = 25). h–i Cell lines, which are normally grown in normoxic conditions (21% oxygen), were transferred into 5% oxygen and centrosome amplification (h) and micronuclei frequencies (i) were estimated. Results depict unpaired two-sided Wilcoxon tests. Boxplots show 25th, 50th and 75th centiles; whiskers indicate 75th centile plus 1.5 × inter-quartile range and 25th centile less 1.5 × inter-quartile range. Notches on boxes extend 1.58 × inter-quartile range/sqrt(n) approximating to the 95% confidence interval for comparing medians. Experiments were performed in four biological repeats for each cell line (n = 8), and four individual imaging fields were analysed for each repeat of each cell line. Data plotted shows the mean across all repeats. Source data are provided as a Source Data file.