Fig. 1: The Usp46 complex promotes expression of the Wingless target gene senseless (sens) in the larval wing disc. | Nature Communications

Fig. 1: The Usp46 complex promotes expression of the Wingless target gene senseless (sens) in the larval wing disc.

From: The USP46 deubiquitylase complex increases Wingless/Wnt signaling strength by stabilizing Arrow/LRP6

Fig. 1: The Usp46 complex promotes expression of the Wingless target gene senseless (sens) in the larval wing disc.The alternative text for this image may have been generated using AI.

A–L RNAi constructs targeting each Usp46 complex component or the yellow negative control were expressed in the posterior compartment (marked by Engrailed (En, green)) of the third instar larval wing discs using the hedgehog (hh)-Gal4 driver. Senseless (Sens, magenta). DAPI (blue) marks nuclei. Scale bar (A–L): 20 µM. Dorsal, top and posterior, right. A–C hh-Gal4-driven expression of a control RNAi construct targeting the yellow (y) gene. No loss of Sens was observed. D–L hh-Gal4-driven expression of RNAi constructs targeting Usp46 (D, F), Wdr20 (G, I) and Uaf1 (J, L) results in decreased Sens in the posterior compartment. Only one RNAi line targeting Uaf1 was available. M Quantification is shown as percentage of discs of each genotype with decreased Sens. N is the number of discs analyzed. ****p < 0.0001 (0 for all genotypes, one-tailed t-test). Source data are provided in the Source Data file.

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