Fig. 6: Arrow expression rescues loss of Sens resulting from RNAi-mediated depletion of Usp46 complex components. | Nature Communications

Fig. 6: Arrow expression rescues loss of Sens resulting from RNAi-mediated depletion of Usp46 complex components.

From: The USP46 deubiquitylase complex increases Wingless/Wnt signaling strength by stabilizing Arrow/LRP6

Fig. 6: Arrow expression rescues loss of Sens resulting from RNAi-mediated depletion of Usp46 complex components.The alternative text for this image may have been generated using AI.

A–H hh-Gal4-driven expression in third instar larval wing discs of RNAi constructs targeting Usp46 (A, C) and Wdr20 (E, G) results in decreased Sens (magenta) in the posterior compartment (marked by Engrailed (En, green)). Sens expression is rescued by co-expression of Arrow (B, D, F, H). GFP-lacZ is expressed in control discs (A, C, E and G). DAPI (blue) marks nuclei. Scale bar: 50 µM. Dorsal, top and posterior, right. I Quantification is shown as percentage of discs of each genotype with decreased Sens. N is the number of discs analyzed. *p < 0.05 (0.022), **p < 0.01 (p values in order: 0.006, 0.0041, and 0.002, one-tailed t-test). Source data are provided in the Source Data file.

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