Fig. 9: Usp46 complex inactivation reduces Arrow levels in a cell autonomous manner.

A–D arr² null mutant clones (green) in the adult posterior midgut. Upon Arrow loss, the Arrow signal intensity is decreased at the cell membrane and in the cytoplasm, confirming the specificity of the Arrow antisera. Additionally, cytoplasmic Arm (yellow) is reduced in Wingless-responding cells upon Arrow loss. E–G Usp46¹ null mutant clones (magenta) in the adult posterior midgut. When Usp46 is inactivated, the levels of cell membrane-associated and cytoplasmic Arrow (green) are decreased cell-autonomously, as is cytoplasmic Arm (yellow). H–J Wdr20³³ null mutant clones (magenta) in the adult posterior midgut. Both Arrow (green) and cytoplasmic Arm (yellow) are decreased cell autonomously when Wdr20 is inactivated. Some cells also display a non-autonomous decrease in cell membrane-associated Arm. K–M Uaf1⁴ null mutant clones (magenta) in the adult posterior midgut. When Uaf1 is inactivated, the levels of cell membrane-associated and cytoplasmic Arrow (green) are decreased cell-autonomously. Some cells also display a reduction in membrane-associated Arm (yellow). DAPI (blue) marks nuclei. Scale bars (A-D) and (E-M): 20 µM (N, O) Quantification is shown as percentage of clones of each genotype with decreased Arrow (N) or Arm (O). N is the number of clones analyzed, *p < 0.05 (0.043 for Uaf1⁴ in O), ****p < 0.0001 (0 for all genotypes in N, 0 for Wdr20³³ and 1E-5 for Usp46¹ in O, one-tailed t-test). Source data are provided in the Source Data file.