Fig. 3: FY mutants are defective in LTA synthesis.
From: TerC proteins function during protein secretion to metalate exoenzymes
a Immunoblot detection of LTA with anti-LTA monoclonal antibodies. Note that in ltaS mutants the signal in the ~15–20 kDa range is absent, and instead longer polymers are detected that depend on the LtaSa enzyme51. The lower inset shows an immunoblot for cells grown in LB + 50 μM Mn. The images are representative of two independent experiments with gels loaded with extracts from equal cell numbers as judged by OD600. Protein molecular weight markers are indicated on left under kDa. b Defects in LTA synthesis activate the σM-dependent cell envelope stress response as monitored using a luciferase transcriptional reporter fusion (PsigM-luxABCD). Cells were grown in LB broth with or without Mn (50 µM) or Ca (50 µM). Data are from three independent experiments and shown with mean ± standard deviation, n = 3. c Defective activation of LTA synthase enzymes is associated with increased sensitivity to compound 1771, an LtaS inhibitor58, 105. Aerobic growth of different strains (WT, FY, ltaS) in LB broth with or without 3 µM 1771 is shown. Data are representative of three independent experiments and are presented as mean ± standard deviation with the sample number n = 2. Additional results, showing the effects of metal supplementation are in Supplementary Fig. 9. Source data are provided as a Source Data file.
