Table 1 Membrane Proteins that Co-immunoprecipitate with MeeF and MeeY^a

From: TerC proteins function during protein secretion to metalate exoenzymes

MeeF-FLAG (heating)^b	MeeY-FLAG (heating)^b	MeeY-FLAG (pH)^c	Functional annotation
			Holotranslocon Proteins
SecY			Subunit of the SecYEG preprotein translocase
SecDF	SecDF	SecDF	PMF-dependent holotranslocon subunit
YrbF			Binds to SecDF as part of the holotranslocon (E. coli YajC ortholog; 34% identity)
			Secretosome and Secretion-related Functions
PrsA	PrsA	PrsA	post-translocation molecular chaperone
HtpX			Quality control membrane protease
FtsH	FtsH		Quality control membrane protease
AtpA	AtpA	AtpA	ATP synthase (subunit alpha); intact ATP synthase interacts with SecYEG translocon⁴³
AtpG	AtpG		ATP synthase (gamma subunit)
	AtpF		ATP synthase, part of the Fo complex
		AtpD	ATP synthase, part of the F1 complex (subunit beta)
			Other Membrane Proteins
FloT			Flotillin, membrane-associated scaffold protein
FloA	FloA		Flotillin, membrane-associated scaffold protein
MalA			6-phospho-alpha-glucosidase
GlcD			possible glycolate oxidase subunit
NupN			lipoprotein, part of guanosine transporter
SwrC	SwrC		resistance-nodulation-cell division (RND)
SsdC			spore shape determinant C (mother cell)
OppF	OppF		Oligopeptide ABC transporter
OppB			Oligopeptide ABC transporter
FrlO			Aminosugar ABC transporter
SdhA	SdhA		succinate dehydrogenase (flavoprotein subunit)
FhuD			hydroxamate siderophore ABC transporter
	QoxA		cytochrome aa3 quinol oxidase (subunit II)
QoxB			cytochrome aa3 quinol oxidase (subunit I)
MsmX			multiple sugar ABC transporter (ATP-binding protein)
FtsA			cell division protein, member of the divisome
YknW	YknW		modulator of ABC transporter assembly, SdpC secretion
		MgtE	primary magnesium transporter

^aOnly integral membrane proteins are listed here. The interacting proteins for MeeF and MeeY include 12 of the 40 most abundant membrane proteins, as measured previously⁵³, consistent with a physiologically relevant association with the secretosome complex.
^bImmunoprecipitated fractions were eluted by heating, samples were treated with 1% TritonX-100 and their western analysis is shown in Supplementary Fig. 6b.
^cImmunoprecipitated fractions were eluted by glycine (pH 3). The MeeY-FLAG sample is shown in Supplementary Fig. 6c.

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Table 1 Membrane Proteins that Co-immunoprecipitate with MeeF and MeeY^a

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