Fig. 6: Arpin is involved in HIV-1 particle release from infected Jurkat T lymphocytes.
From: HIV-1 diverts cortical actin for particle assembly and release
A Anti-Arpin and anti-p24 immunoblots of VSV-G pseudotyped HIV-1(i)GFP∆Env infected Jurkat CD4+ T lymphocytes cell extracts with siArpin (+) or siControl (-) and the corresponding purified released virus. GAPDH is used as a loading control. B Histogram showing the percentage (%) of Arpin gene expression after siRNA treatment corresponding to the Western blot in A) (N = 3 independent experiments). C Histogram showing the percentage of viral release calculated from HIV-1 Gag(i)GFP and HIV-1 CAp24 release observed in the Western blot in A), N = 3 independent experiments, one-tailed Mann Whitney to test for decrease in particle release. D Histogram showing the decrease in viral release (25 ± 3%) upon siArpin treatment normalized to the control (CTRL), N = 3 independent experiments, one-tailed Mann–Whitney to test for the decrease in particle release. Data are presented as mean values +/- SEM. Exact p-values are given in graphs. Source data are provided as Source data files.
