Fig. 6: Tissue reaction to TTX formulations.
a, b Cytotoxicity of formulations to C2C12 (c) and PC12 (d) cells. Black up-pointing triangle, navy down-point triangle, green square, pink diamond, and orange circle represent PBS, free TTX, liposomes encapsulating TTX (TTX@Lipo), liposomes encapsulating TTX and ICG (ICG + TTX@Lipo) and aromatized liposomes encapsulating TTX (TTX@Lipo-Ph), respectively. Data are presented as mean ± SD, n = 4 independent experiments. Statistical analysis was performed using one-way ANOVA with a Tukey post hoc test. c Representative photographs of the sites of injection upon dissection 4 days after injection. The green color is due to ICG. Arrows indicate the locations of formulations. d Representative H&E-stained sections of nerves and surrounding tissues, and toluidine blue-stained section of nerves. The scale bar is 100 µm for H&E-stained sections; 20 μm for toluidine blue.
