Fig. 1: Identification of the thermostable type I-B CRISPR-Cas from P. thermoglucosidasius.
From: A thermostable type I-B CRISPR-Cas system for orthogonal and multiplexed genetic engineering
a Genetic architecture of the Type I-B and III-B CRISPR-Cas operon. The diamond: repeat sequence. The square: spacer sequence. b Protospacers predicted by web CRISPRTarget tool. The yellow sequences: spacers from genome. The black sequences: protospacer sequence. The potential PAM is indicated by a blue background. c Sequence alignment of seven repeats from Type I-B and Type III-B CRISPR-Cas system. Non-conservative sites are highlighted in red. d Plasmid interference activity of the type I-B CRISPR-Cas system in strain NCIMB 11955. The transformation efficiency was indicated by the rate of recovered clones. A low-transformation efficiency indicates a high plasmid interference activity. e Plasmid interference activities of the type I-B CRISPR-Cas system at different temperatures in strain NCIMB 11955. f Plasmid interference activities of the type I-B CRISPR-Cas system at 37 °C in E. coli. Data represented three biological repeats and displayed as mean ± SD. Source data are provided as a Source Date file.
