Fig. 2: Identification of FAM210A as a cold-inducible mitochondrial inner membrane protein in BAT.
From: FAM210A is essential for cold-induced mitochondrial remodeling in brown adipocytes
a The list of differentially expressed mitochondrial proteins with domain of unknown function (DUF) (One-way ANOVA). b Quantification of FAM210A expression in mitochondrial proteomic data (n = 3 mice per group; mean ± s.e.m; two-tailed unpaired Student’s t test; P = 0.0497, 0.006, 0.0044, and 0.0011). c Immunoblotting analysis of FAM210A in BAT from mice upon cold exposure at different time points (n = 3 mice per group). d Schematic showing the predicated domain structure and RoseTTAfold prediction of FAM210A. MTS, mitochondrial targeting sequence; TM, transmembrane region; DUF1279, domain of unknown function 1279; CC, coiled-coil domain. e Immunoblotting analysis of FAM210A in brown adipocytes (BAs) during differentiation (n = 3 independent experiments). f Immunofluorescence showing the colocalization of FAM210A with mitochondria in BAs before and after differentiation (n = 3 independent experiments; scale bars, 10 µm). g Single-molecule localization image of overexpressed Fam210a in Cos-7 cells (n = 3 independent experiments; scale bars, 2 µm). h Protease protection assay with mitochondrial fractions purified from BAT mitochondria (PK, proteinase K; n = 3 independent experiments). *P < 0.05, **P < 0.01. Source data are provided as a Source Data file.
