Fig. 5: Fam210a is required for cristae and mitochondrial remodeling in BAT.
From: FAM210A is essential for cold-induced mitochondrial remodeling in brown adipocytes
a Representative images and quantification of isolated mitochondria pellets from control (Ctrl) and Fam210aAKO (AKO) BAT (n = 6 mice per group; mean ± s.e.m; two-tailed unpaired Student’s t test; P = 0.0000003729). b Mitochondria copy number in BAT, iWAT, and gWAT from control and Fam210aAKO mice at room temperature (RT) (n = 4 mice for control BAT; n = 5 mice for control iWAT and gWAT; n = 4 mice for Fam210aAKO; mean ± s.e.m; two-tailed unpaired Student’s t test; P = 0.010882). c Mitochondria copy number in BAT, iWAT, and gWAT from control and Fam210aAKO mice upon cold exposure (CT) for 7 d (n = 5 mice per group for BAT and iWAT; n = 4 mice per group for gWAT; mean ± s.e.m; two-tailed Student’s t test; P = 0.000395 and 0.049103). d Representative TEM images of BAT and iWAT from control and Fam210aAKO mice at RT and cold exposure for 7 d (n = 3 mice per group; for 6000X, scale bars, 2 µm; for 16000X, scale bars, 1 µm). e Quantification of cristae number per mitochondrion shown in (d) (n = the number of mitochondria counted in the order they appear on the graph from left to right: 156, 104, 431, 487, 459, 76; 3 mice were used for each group; mean ± s.e.m; two-tailed unpaired Student’s t test; ****P < 0.00000001). f Schematic diagram of the strategy used to generate inducible adipocyte-specific Fam210a knockout mice (Fam210aiAKO). g Schematic showing timing of tamoxifen (TMX) induction, cold exposure, and sampling for control and Fam210aiAKO mice (diagram created with BioRender.com). h Representative TEM images of control and Fam210aiAKO BAT upon cold exposure for different times (n = 3 mice per group; scale bars, 1 µm). i Quantification of Class 3 mitochondria in (h) (n = 37, 42, 34, and 24 images for the control group at 0 h, 6 h, 3 d, and 7 d after cold, respectively; n = 41, 38, 49, and 34 images for Fam210aiAKO group at 0 h, 6 h, 3 d, and 7 d after cold, respectively; the number of mitochondria counted in the order they appear on the graph from left to right: 510, 620, 781, 559, 685, 861, 526, 521; 3 mice were used for each group; mean ± s.e.m; two-tailed unpaired Student’s t test; ****P < 0.00000001). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Source data are provided as a Source Data file.
