Fig. 4: Identification and characterization of exMT-enriched CD36 fraction. | Nature Communications

Fig. 4: Identification and characterization of exMT-enriched CD36 fraction.

From: Release of CD36-associated cell-free mitochondrial DNA and RNA as a hallmark of space environment response

Fig. 4

a Screening of an antibody panel against cell-surface markers. Antibodies from four 96-well plates were pooled and mixed with in-flight or post-flight astronaut plasma samples. Created with BioRender.com. b Purified DNA from each immunopurification was analyzed by real-time PCR using the MT-ND1 and globin (HGB) gene. Red arrows indicate lower PCR cycle threshold (Ct) values, indicating the specific recovery of mtDNA from flight plasma samples (#56 and #63) but not from post-flight samples (#47), through antibody pool G. c Second round of antibody screening in which antibodies contained in pool G were subdivided into 6 pools. Pool 2 specifically enriched mtDNA from in-flight samples (#52 and #55) but not from post-flight samples (#47). d Third round of antibody screening, which identified the anti-CD36 antibody. e Heatmap of differentially purified RNAs between anti-CD36 immunopurification (IP) and input plasma. IP was performed using three different in-flight plasma samples. f Tissue enrichment analysis of the 406 CD36-enriched genes against the Human Protein Atlas Tissue Gene Expression Database using DAVID software. g RNA expression profile for the 406 CD36-enriched genes obtained from the Genotype-Tissue Expression (GTEx) database.

Back to article page