Fig. 1: KRAS-G12V-9 specific TCR screening and binding validation in HEK-293T cells.
From: KRAS G12V neoantigen specific T cell receptor for adoptive T cell therapy against tumors
a TCR repertoire analysis of cloned KRAS-G12V-9/HLA-A*11:01 tetramer+ CD8+ T cells from Mus-T5, Mus-TF1, Mus-TF2, and Mus-TF6. Numbers below the pie charts represent the number of TCRs identified in the mouse. b The expression of TCRs in HEK-293T cells after co-transfection of chimeric TCR and human CD3-CD8 constructs was detected by staining with antibodies specific to human αβ TCR. Binding of wildtype KRAS-G12wt-9/HLA-A*11:01 tetramer (c) or mutated KRAS-G12V-9/HLA-A*11:01 tetramer (d) to HEK-293T cells transiently expressing the indicated TCRs as in (b) for each panel. The Y axis represents the staining events by the indicated tetramer, while the X axis represents the staining events of CD3-positive cells by the anti-CD3 antibody. Data in (b), (c), and (d) are representative of three independent experiments.
