Fig. 2: Two candidate loci for proton translocation. | Nature Communications

Fig. 2: Two candidate loci for proton translocation.

From: Structural basis for triacylglyceride extraction from mycobacterial inner membrane by MFS transporter Rv1410

Fig. 2

a Side view of MHAS2168. MHAS2168 color scheme same as in Fig. 1b. Point mutation sites are shown as colored sticks. b Enlarged periplasmic top view of MHAS2168 with mutated residues shown as colored sticks and interacting residues mentioned in the text as grey sticks, both with heteroatom depiction. Hydrogen bonds are shown as light blue dashed lines. c Vancomycin sensitivity assays in M. smegmatis dKO cells, complemented with empty vector control (EV), wild-type LprG/Rv1410 operon (WTMtb), or mutant operons containing unaltered LprG (Rv1411) and mutated transporter Rv1410 as indicated. d Analogous analysis as in (c), with M. smegmatis dKO cells expressing instead wild type MHAS2167/68 operon (WTMH) or mutant operons containing unaltered LprG (MHAS2167) and mutated transporter MHAS2168 as indicated. The growth curves in (c) and (d) are representative of three biological replicates and data are presented as mean values +/- SD of four technical replicates.

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