Fig. 1: Cryo-EM structures of Organic Cation Transporter 1 in the inward-open conformation.
From: Structural basis of promiscuous substrate transport by Organic Cation Transporter 1
OCT1 consists of four domains: N-terminal lobe colored in blue, C-terminal lobe colored in orange, extracellular domain (ECD) colored in green, and intracellular helices (ICH) colored in pink. a Cryo-EM map of OCT1-apo. b Backbone structure of OCT1-apo with domains labelled. c–g Cryo-EM maps of OCT1-apo and OCT1 after incubation with thiamine (OCT1-THA), metformin (OCT1-MTF), fenoterol (OCT1-FNT), and diltiazem (OCT1-DTZ). Top: cross-section of map perpendicular to the membrane, to show binding site (grey) relative to the structure (maps contoured to 9 σ). Bottom: close-up of binding site, with map around substrate shown as blue mesh (maps contoured to σ = 7.5). Further detailed views of binding site map density can be found in Supplementary Fig. 7.
