Table 1 Primers used for cloning
From: ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling
Name | Sequence 5′–3′ |
|---|---|
ARF1-Q71Lfwd | TTG GAC AAG ATC CGG CCC CTG TGG |
ARF1-Q71Lrev | GCC ACC CAC GTC CCA CAC AGT |
ARF1-T31N-fwd | AAT ACG ATC CTC TAC AAG CTT AAG CTG GGT GAG A |
ARF1-T31N-rev | CTT CCC TGC AGC ATC CAG GCC |
ARF1 R99Cfwd | GAG TGT GTG AAC GAG GCC CGT GAG GAG C |
ARF1 R99Crev | TCT GTC ATT GCT GTC CAC CAC GAA GAT C |
SDM-ARF1-R99K/E/A R | TTG CTG TCC ACC ACG AAG |
SDM-ARF1-R99A-F | TGA CAG AGA GGC TGT GAA CGA GGC CC |
SDM-ARF1-R99E-F | TGA CAG AGA GGA GGT GAA CGA GGC C |
SDM-ARF1-R99K-F | TGA CAG AGA GAA GGT GAA CGA GGC C |
STING-R238A/Y240A-fwd | CAT CAA GGA TGC GGT TGC CAG CAA CAG CAT C |
STING-R238A/Y240A-rev | CCA GCA CGG TCA GCG GTC TG |
STING-S366A-fwd | GCT CCT CAT CGC TGG AAT GGA AAA GCC C |
STING-S366A-rev | TCA GGC TCT TGG GAC ATC |
VSVG(tsO45)-KDELR-fwd | GAC TTG GAA ACA GAA TTC TGA TGG CCA TGA ACA TTT TCC G |
VSVG(tsO45)-KDELR-rev | CTC ACC ATT GGA TCC CGG GCC CCT GCT GGC AAA CTG AGC TTC T |
ARF1-TagRFP-fwd | GTC CAA TCA GCT CCG GAA CCA GAA GGC GGT GTC TAA GGG CGA AG |
ARF1-TaqRFP-rev | CAG CTA TGA CCG CGG CCG GCC GT T TTT AAT TAA GTT TGT GCC CC |
pBOB-ARF1-fwd | CCT CCA TAG AAG ACA CCG ACT CTA GAG CCA CCA TGG GGA ACA TCT TCG CC |
pBOB-ARF1-rev | CTA TGA CCG CGG CCG GCC GTT TAA ACC TTA TCG TCG TCA TCC |
pBOB-IRES-Puro-fwd | ACG GCC GGC CGC GGT CAT AGG CGG CCG CTC TAG CCC AAT TCC |
pBOB-IRES-Puro-rev | GCT CCA TGT TTT TCC AGG TTT TCA GGC ACC GGG CTT GCG |
EGFP-STING-fwd | ATT ACT CGA GAT GCC CCA CTC CAG |
EGFP-STING-rev | GAA TTC TCA AGA GAA ATC CGT GCG GA |
