Fig. 4: NTCP is required for GDCA- or TDCA-mediated cholesterol transport in cells. | Nature Communications

Fig. 4: NTCP is required for GDCA- or TDCA-mediated cholesterol transport in cells.

From: Bile acids-mediated intracellular cholesterol transport promotes intestinal cholesterol absorption and NPC1L1 recycling

Fig. 4: NTCP is required for GDCA- or TDCA-mediated cholesterol transport in cells.The alternative text for this image may have been generated using AI.

a Confocal images of CRL1601/NPC1L1-3×Myc-EGFP cells pretreated with vehicle, GDCA or TDCA. Scale bar, 10 μm. NC: negative control. Percentages of ERC cholesterol (b) and relative intensity of surface NPC1L1 (c) in (a) were quantified. The average intensity of surface NPC1L1 in GDCA-treated control cells was defined as 1. Values were presented as mean ± SD (n = 3 independent trials, 100 cells/trial). Two-way ANOVA with Tukey post hoc test, ***P < 0.001; ns, no significance. d Percentages of cholesteryl esters relative to total cholesterol in NC or Ntcp knockdown CRL1601/NPC1L1-3×Myc-EGFP cells with a 2-h incubation of indicated BAs. Values were presented as mean ± SD (n = 3). Two-way ANOVA with Tukey post hoc test, ***P < 0.001; ns, no significance. e LipidTox staining of CRL1601/NPC1L1-3×Myc-EGFP cells after 2-h incubation of vehicle, GDCA or TDCA. Scale bar, 10 μm. f Relative intensity of LipidTox in (e) was quantified. The average intensity of GDCA-treated control cells was defined as 1. Values were presented as mean ± SD (n = 3 independent trials, 100 cells/trial). Two-way ANOVA with Tukey post hoc test, ***P < 0.001; ns, no significance. g D4H staining of CRL1601/NPC1L1-3×Myc-EGFP cells with 2-h incubation of vehicle, GDCA or TDCA. Scale bar, 10 μm. h Relative intensity of D4H in (g) was quantified. The average intensity of D4H in GDCA-treated control cells was defined as 1. Values were presented as mean ± SD (n = 3 independent trials, 100 cells/trial). Two-way ANOVA with Tukey post hoc test, ***P < 0.001; ns, no significance. i Cholesterol absorption of the vehicle or linerixibat-treated mice was measured by dual isotope ratio method. Values were presented as mean ± SD (n = 4 mice). Unpaired two-tailed Student’s t test. **P < 0.01. j Deparaffinized sections of the ileal intestine from the vehicle or linerixibat-treated nine-week-old NPC1L1-EGFP male mice (n = 2). Scale bar, 10 μm. k Filipin staining of ileal intestine sections from nine-week-old C57BL6J mice (n = 2). Scale bar, 10 μm (main); 1 μm (inset). Chol: Cholesterol. Source data are provided as a Source Data file.

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