Fig. 2: Decreases of the SUN1/2 protein levels in response to inflammatory cues.
From: SUN1/2 controls macrophage polarization via modulating nuclear size and stiffness
a Expression levels of the indicated proteins in PEMs that were challenged with LPS for various periods of time. b Flow cytometry analysis of SUN2 expression in F4/80+ PEMs after LPS stimulation for 5 h. c Immunofluorescence analysis of α-Tubulin (green), Phalloidin (Red), Sun1 (purple) and DAPI (blue) in hBM after LPS stimulation. LPS, 100 ng/ml. Scale bar, 5 μm. d Fluorescence microscopy of SUN2 and LaminA/C in LPS-stimulated macrophages (n = 10 cells per group). e A schematic model of SUN-mediated alterations to the structure of the macrophage during LPS-induced activation. Representative of 2 independent experiments (a, c). Data were presented as means ± SD (d). Two-sided Tukey post-hoc test was used to compared differences between groups after One-way ANOVA (d). *p < 0.05; ***p < 0.001, n.s no significance (p > 0.05) in comparison with control group. See also Supplementary Fig. 2.
