Fig. 4: Ultrastructural alteration of mitochondria of Pkhd1 KO kidney tubules.

a–d Electron micrographs of WT and Pkhd1 KO kidney proximal tubules. a WT proximal tubule identified by brush border (BB) at the center of the lumen. Scale bar 2 μm. The area outlined in the white box is magnified in (b). b Magnified area of WT image. Scale bar 500 nm. c KO proximal tubule identified by brush border (BB) at the center of the lumen. Scale bar 2 μm. The area outlined in the white box is magnified in (d). d Magnified area of KO image. Scale bar 500 nm. e–h Graphs depicting shape descriptors. e Area, (f) Perimeter, (g) Circularity, and (h) Solidity. A total of 962 mitochondria were measured from 3 kidneys, using images from 3–5 proximal tubules per kidney. Points on each graph represent the average for each kidney measured and the bar represents the average of all mitochondria measured. Error bars represent the standard error of the mean (SEM). P-values were derived by Student’s t-test. i–l Electron micrographs of mitochondria in WT and Pkhd1 KO kidney proximal tubules. i WT mitochondria. Scale bar 100 nm. k Magnification of WT cristae. Scale bar 100 nm. j KO mitochondria. Scale bar 100 nm. l Magnification of KO cristae. Scale bar 100 nm. m Graph shows average cristae diameter in nm. Each point represents the average diameter of cristae in a single mitochondrion. 50 mitochondria measured per genotype. Error bars represent SEM. P-values were derived by Student’s t-test. Source data are provided as a Source Data file.