Fig. 1: V-HARP1 gets into pavement and mesophyll cells and locates in endosomes.
From: Endocytosis-mediated entry of a caterpillar effector into plants is countered by Jasmonate
The wounded plant leaves of wild-type (a), or of mCherry-CLC2, Rha1-mCherry and VHA-a1-mRFP transgenic Arabidopsis (b) were incubated with V-HARP1, respectively. a Transmission electron microscope (TEM) observation of V-HARP1 in pavement and mesophyll cells. Ultrathin sections from V-HARP1 treated leaves were immune-gold labeled with anti-GFP antibody. The enlarged view of the red boxed regions (1 and 2) from the observed pavement and mesophyll cells was shown independently. The arrows indicate the immune-gold labeled V-HARP1. Scale bars were indicated in each image. b V-HARP1 colocalized with the mCherry-CLC2 labeled clathrin-coated vesicles, the VHA-a1-mRFP labeled secretory vesicles and the Rha1-mCherry labeled prevacuolar compartment/multivesicular body or late endosomes. The inset panels highlighted the dotted square regions. Scale bar: 2 µm for inset and 10 µm for others. c Fluorescence intensity (in arbitrary units, arb. units) in cross-section [dotted line in inset (b)]. The orange indicate the intensities of V-HARP1, and the bluelines indicate the intensities of CLC2, Rha1 and VHA-a1. d Quantification of CLC2, Rha1 or VHA-a1 colocalized V-HARP1. Ratio of the colocalized to total V-HARP1 granules in (b) were calculated. Data are mean ± SEM (n = 6), 6 leaves from 3–5 independent plants were examined. Source data are provided as a Source Data file.
