Fig. 3: Deficient V-HARP1 import is companied with the reduced effector activities.
From: Endocytosis-mediated entry of a caterpillar effector into plants is countered by Jasmonate
a, b V-HARP1 was less imported into ctl1, sld1 sld2, patl2 and tet8. Leaves of the wild type (Col-0) and indicated mutants were wounded and incubated with V-HARP1. Scale bar, 200 µm. c Injected V-HARP1 in plants were observed by confocal microscopy. V-HARP1 (0.2 μg/ml) was injected into wild type (Col-0), tet8, ctl1 and patl2. Scale bar, 5 µm. d Quantification of the V-HARP1 granules in (c). The leaves were selected to count the number of V-HARP1 granules. Data are mean ± SEM (n = 6 biological replicates) and analyzed by one-way ANOVA with two-sided Dunnett’s post hoc test (****p < 0.0001). e–g V-HARP1 reduced wounding response in wild type (Col-0) but not in ctl1 (e), patl2 (f) and tet8 (g). Plant leaves were wounded and quickly applied with 1 mg/ml Venus (W+Venus, blue indicated) or V-HARP1 (W + V-HARP1, green indicated) proteins on the wounding sightes, respectively. Gene expressions 4 h post treatments were detected by qRT-PCR. The gene expressions in the unwounded plants (untreated, gray indicated) were set to 1. Data were analyzed by two-way ANOVA followed by multiple comparisons with two-sided Fisher’s LSD test (n.s: not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). Data are mean ± SEM (n = 4 biological replicates in e, g n = 3 biological replicates in f). Source data are provided as a Source Data file.
