Fig. 5: JA negatively regulates plant endocytosis.
From: Endocytosis-mediated entry of a caterpillar effector into plants is countered by Jasmonate
a–c JA inhibits the expressions of genes related to secretary vesicles and membrane. Ten-day-old Arabidopsis (Col-0) and aos were wounded and leaves were harvested 2 h post wounding (W). The untreated plant leaves were used as control. Total RNAs were extracted for RNA sequencing and qRT-PCR analysis. a GO enrichment of the genes with expression significantly higher in aos than those in wild type (Col-0) after wounding (aos_W > Col-0_W). b The ClueGO network of the enriched items as described in (a). c qRT-PCR of the selected genes (aos_W > Col-0_W) according to the RNA-seq data. The gene expressions in the unwounded wild type (Col-0) (untreated) were set to 1. Data are mean ± SEM (n = 3 biological replicates) and analyzed by two-way ANOVA followed by multiple comparisons with two-sided Fisher’s LSD test. (*p < 0.05, **p < 0.01, ***P < 0.001, ****P < 0.0001). Blue and yellow box indicate untreated and wounding (W) treatment, respectively. d JA suppressed the accumulations of internalized endosomes. The wounded leaves of Col-0 and aos were incubated with 50 µM MeJA (W + JA) or Ethanol (W) solutions and further stained with FM4-64 to trace the internalized endosomes. Pavement cells were observed under microscopy. Scale bar, 5 µm. Source data are provided as a Source Data file.
