Table 1 One- and two-photon photophysical parameters of eLACCO2.1
From: Lactate biosensors for spectrally and spatially multiplexed fluorescence imaging
l-Lactate | eLACCO2.1 | eLACCO1.1 (ref. 9) | iLACCO1 (ref. 19) | jGCaMP8s (ref. 48) | EGFP | ||||
|---|---|---|---|---|---|---|---|---|---|
— | + | — | + | — | + | —a | +a | ||
Relative fraction of neutral chromophore (ρN) | 0.97 | 0.24 | 0.97 | 0.57 | 0.97 | 0.79 | 0.74b | 0.17b | 0.07c |
Relative fraction of anionic chromophore (ρA) | 0.03 | 0.76 | N/A | 0.43 | N/A | 0.21 | 0.26b | 0.83b | 0.93c |
Neutral extinction coefficient (mM−1 cm−1, εN) | 42 | 45 | 34 | 38 | 39 | 39 | N/A | N/A | N/A |
Anionic extinction coefficient (mM−1 cm−1, εA) | 76 | 65 | 78 | 89 | 81 | 74 | 2.1 | 57 | 56.0d; 58.3e |
Neutral quantum yield (φN) | 0.06 | N/A | 0.20 | 0.23 | N/A | N/A | N/A | N/A | N/A |
Anionic quantum yield (φA) | 0.47 | 0.82 | 0.60 | 0.78 | 0.72 | 0.84 | 0.47 | 0.53 | 0.67d; 0.76e |
Molecular brightness (ρA × εA × φA) | 1.1 | 41 | 4.5f | 30 | 0.74 | 13 | 0.26 | 25 | 34.9d; 41.2e |
Neutral one-photon absorbance peak (nm) | 395 | 398 | 398 | 397 | 400 | 400 | N/A | N/A | N/A |
Anionic one-photon absorbance peak (nm) | 493 | 494 | 493 | 496 | 492 | 492 | N/A | N/A | 488d |
Two-photon cross section (GM) (position/nm) | N/A | 32 (932) | N/A | 48 (924) | N/A | 42 (928) | N/A | 25 | 54 (911)e |
Two-photon brightness F2 (GM) (position/nm)g | 0.88h (932) | 20 (932) | 2.2 (924) | 16 (924) | 0.52 (928) | 7.3 (928) | N/A | N/A | 41 (911)e |