Fig. 6: Transcriptomic shifts under doxycycline treatment. | Nature Communications

Fig. 6: Transcriptomic shifts under doxycycline treatment.

From: Miniature spatial transcriptomics for studying parasite-endosymbiont relationships at the micro scale

Fig. 6: Transcriptomic shifts under doxycycline treatment.The alternative text for this image may have been generated using AI.

A Violin plots of the B. malayi UMIs and unique genes per capture spot across different treated worm tissue sample sections. B Scatter plot of the average B. malayi gene expression between consecutive treated worm sections (T1_4 and T1_5) across all genes (r = 0.92, p < 0.05). The statistical test is based on the Pearson’s product moment correlation coefficient (r) with 95% confidence intervals and p-values (p) were estimated with two-sided alternative hypothesis. C Violin plots of the Wolbachia unique molecules (UMIs) per spot and unique genes per spot in control and treated worm sample sections. D Violin plots of the B. malayi unique molecules (UMIs) per spot and unique genes per spot in control and treated worm sample sections in Wolbachia + (W + ) and Wolbachia- (W-) spots. E Dotplot depicting the expression of select B. malayi genes in Wolbachia+ spots in treated worms (W + _T), Wolbachia+ spots in control worms (W + _C), Wolbachia- spots in treated worms (W-_T), and Wolbachia- spots in control worms (W-_C). Average expression values indicate the average UMI counts (normalized) per spot across all spots in each cluster. Percent expressed values indicate the percentage of spots within each cluster that express each gene. F 2D images of Bma-snx-1 and Bma-ned-8 gene expression in sample section T2_1 between Wolbachia + (W + ) and Wolbachia- (W-) spots. Magnification 20x.

Back to article page