Fig. 4: Altered transcriptional profiles in Alkbh5−/− oocytes. | Nature Communications

Fig. 4: Altered transcriptional profiles in Alkbh5−/− oocytes.

From: ALKBH5 controls the meiosis-coupled mRNA clearance in oocytes by removing the N 6-methyladenosine methylation

Fig. 4: Altered transcriptional profiles in Alkbh5−/− oocytes.The alternative text for this image may have been generated using AI.

a Flow chart of SMART-seq. 20 GV, BD and MII oocytes from WT and Alkbh5−/− females are collected at 0, 4 and 16 h respectively, and subjected to sequencing as detailed in “Methods”. Two biological independent samples are reported. b Heatmap showing gene expressions in WT and Alkbh5−/− oocytes. Each row represents one gene and each column represents one sample. FPKM of each gene are normalized to Z-score indicated by color scale. c PCA clustering of sequenced samples. Each dot represents an individual library, labeled with genotype and development stage. d The relative expression levels of Alkbh5 and Atcb in GV, BD and MII oocytes. n = 2 biological replicates. Data are presented as mean ± SD. e Comparison of total RNA levels in WT and Alkbh5−/− SMART-seq libraries at each indicated stage. n = 2 biological replicates. Data are presented as mean ± SD. f Volcano plots showing differentially expressed genes (DEGs) between WT and Alkbh5−/− groups at the GV, BD and MII stages. Each dot represents an individual gene and numbers of DEGs are indicated. Genes differential expression analysis was performed by DESeq2 software between two different groups. DEGs cut-off is false discovery rate (FDR) < 0.05 and absolute fold change≥2. BD, GVBD. g Sankey diagram depicting the dynamic composition of significantly up-regulated, no-differential, or down-regulated transcripts from the GV to MII stages. h Four quadrant diagram showing correlation between maternal transcriptome from the GV to MII stages and gene expressions in Alkbh5−/− MII oocytes with respect to WT MII oocytes. The significantly upregulated genes in Alkbh5−/− MII oocytes that are degraded in GV-MII transition are highlighted in blue. The number of genes in each gate is shown. i Network plot presenting functional enrichment analysis of DEGs at MII stage. Metascape was applied as detailed in “Methods”. The P values are calculated using hypergeometric test of functional annotation with default parameters. Top 10 ranked annotations are reported and their P values are shown. Source data are provided as a Source Data file.

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