Fig. 7: ALKBH5-mediated demethylation ensures the timely decay of maternal RNAs. | Nature Communications

Fig. 7: ALKBH5-mediated demethylation ensures the timely decay of maternal RNAs.

From: ALKBH5 controls the meiosis-coupled mRNA clearance in oocytes by removing the N 6-methyladenosine methylation

Fig. 7: ALKBH5-mediated demethylation ensures the timely decay of maternal RNAs.The alternative text for this image may have been generated using AI.

a Heatmaps showing the expression levels of GV-MII decayed transcripts (abbreviated as “GMD”) in WT oocytes during GV-MII transition. The rightmost columns indicate the m6A marking status of GMD. bBoxplots showing the expression fold changes (left) and RNA abundance (MII stage, right) of m6A-modified and unmodified transcripts in oocytes. n = 2074 (m6A−), 855 (m6A+) transcripts. P = 6.94e−4, 0.000006, 3.03e−9 and 0.000037 (from left to right). c Proportions and numbers of m6A-loss and inherited transcripts in decayed and non-decayed m6A-modified transcripts. P values, two-tailed Fisher’s exact test. P = 0.000074. d Boxplot (left) and dot plot (right) showing the expression fold changes of loss and inherited GMD transcripts in WT and Alkbh5−/− oocytes. The dot represents the mean value of expression fold changes. WT, P = 1.62e-8. e–h RNA immunoprecipitation results showing the relative m6A enrichments among the indicated genes in GV and MII oocytes from WT and Alkbh5−/− mice. The data validated by qPCR are present as mean ± SD and are normalized to WT GV samples. P value, two-tailed Student’s t test. n = 3 biological replicates. i A schematic model of ALKBH5 function during GV-MII transition. j Degradation patterns of loss-GMD transcripts during GV-MII transition. Each green line represents the expression level of an individual gene. The blue and red line represent the median expression levels in WT and Alkbh5−/− oocyte, respectively. k Dot plots showing percentages of repressed decayed genes in loss-GMD (n = 298) and stabilized genes in affected transcripts (n = 237). De. Depressed. l Venn diagram showing the overlapping of loss-GMD with DEGs in Alkbh5−/− MII oocytes. m–p RIP-qPCR analysis showing the fold changes of ALKBH5 binding ability with the indicated RNAs in oocytes. The bar is mean ± SD resulting from three independent experiments. P value, two-tailed Student’s t test. Data in b and d are presented as box and whisker plots. The central line denotes median value, while the bounds of box mark the 25th to 75th percentiles and whiskers refer to minimum and max values by Tukey. P values in b and d are calculated by two-tailed unpaired Wilcoxon test. Source data are provided as a Source Data file.

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