Fig. 6: Gemin3 is important for U1 and U4 localization in Cajal bodies.

a, b Gemin3 was downregulated by RNAi and fluorescently labeled U1 and U4 pre-snRNAs were microinjected into the cytoplasm. Pre-snRNAs with relaxed NSS (noNSS) did not require Gemin3 to reach Cajal bodies. Strengthening NSS prevented Cajal body localization even in cells treated with negative control siRNA. a Cajal bodies marked by arrows were enlarged 3 times and shown in insets. Scale bar represents 5 μm. b Localization of injected snRNAs in Cajal bodies (marked by coilin immunostaining) was monitored, quantified, and shown in box plots. n—number of assayed cells. On each box, the central mark indicates the median, and the bottom and top edges of the box indicate the 25th and 75th percentiles, respectively. The whiskers extend to the most extreme data points not considered outliers, and the outliers are plotted individually using the ‘+‘ symbol.