Fig. 9: Phenotypes of continuous high-fat-diet-fed ieIRKO mice at 18 months of age.

Representative image of liver and ileum (a). Liver sections were stained with HE and Sirius red. Ileal sections were stained with HE and PAS-AB. Scale bar, 10 mm for macroscopic image, 100 μm for microscopic image. Visible tumor number (b) and maximum diameter (c) in liver of each mouse. Plasma α-fetoprotein (d). Relative mRNA expression of InsR (e) and Crps (f). Control (n = 5) and ieIRKO (n = 6) (b, c, d, e, f), *P < 0.05, ****P < 0.0001, 2-sided Mann Whitney’s U test (b, c, d), and 2-sided unpaired t test (e, f). Values of the data are expressed as mean ± SEM (b, c, d, e, f). Source data are provided as a Source Data file. The exact P values are provided in Supplementary Data 3 unless they are below 0.0001.