Fig. 3: Off-target activities of TALE-free DddA homologs on mtDNA. | Nature Communications

Fig. 3: Off-target activities of TALE-free DddA homologs on mtDNA.

From: Developing mitochondrial base editors with diverse context compatibility and high fidelity via saturated spacer library

Fig. 3: Off-target activities of TALE-free DddA homologs on mtDNA.The alternative text for this image may have been generated using AI.

a Off-target numbers on mtDNA induced by TALE-free DddA homologs with different splits. Untreated cells are used as negative control (NC). b Average C/G to T/A conversion rate (%) induced by TALE-free DddA homologs with different splits. c Whole mtDNA off-target plots of DddA11 and three highly active homologs (FZY2, WC03 and Q2L7). Sites with C/G to T/A editing beyond 1% are shown by dots with corresponding colors. d The Venn diagram showing the comparison of edited cytosines induced by TALE-free DddA homologs with different split. e Heatmap of Spearman Correlation Coefficient. The gradation of color represents the correlation of off-target editing features of TALE-free DddA homologs with different splits. f Probability sequence logo of edited cytosines on mtDNA induced by TALE-free DddA homologs with different splits. a–f Values reflect the mean of n = 2 independent biological replicates. Source data are provided as a Source Data file.

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