Fig. 5: AMP effects on transcription and translation processes.
From: Targeting nucleic acid phase transitions as a mechanism of action for antimicrobial peptides
Cell-free eGFP synthesis followed in the absence and presence of antimicrobial peptides (a–f). The cell-free synthesis of eGFP was initiated by supplementing CFPS solution with 6 ng/μL plasmid DNA (a–c) or 80 ng/μL mRNA (d–f) encoding eGFP protein. Continuous lines are the fits (see ‘Methods’). Comparison of EC50 values of AMPs in plasmid DNA- or mRNA-initiated CFPS reaction (g). Data are presented as the best-fit values ± RMSD. AMP vs. Hfq competition assay (h). Case #1: 100 μM P113 was mixed with 250 ng/μL of poly(A) and incubated for 5 min at room temperature. Subsequently, 0.1–300 μM Hfq was introduced and the sample was incubated for an additional 5 min before imaging via a confocal fluorescence microscope. Case #2: 0.1–100 μM Hfq was pre-incubated with 250 ng/μL of poly(A) for 5 min before introducing 100 μM P113. The scale bars are 20 μm. Source data are provided as a Source data file.
