Table 2 L926Q stabilizes the 3HB

From: How myosin VI traps its off-state, is activated and dimerizes

Construct

Fluorescence ratio without actin + ATP

Fluorescence ratio with actin + ATP

Molar ratio of labeling per myosin head

MDIns2/IQ/3HB T845C

256.1 ± 24.4

243.8 ± 14.5

1.03

MDIns2/IQ/3HB T845C, A880C

22.5 ± 5.8

22.6 ± 7.2

2.10

Zippered dimers A880C

238.2 ± 23.7

232.3 ± 30.4

1.11

Zippered dimers T845C, A880C

206.5 ± 19.6

214.3 ± 12.6

2.22

Zippered dimers T845C, A880C, L926Q

147.53 ± 30.4

164.6 ± 18.7

2.14

  1. Fluorescence observed by TMR labeling of one or two cysteine residues inserted into the three-helix bundle of monomers (MDIns2/IQ/3HB) and zippered dimers. Fluorescence was analyzed by a ratio of the emission values to that of the absorption values for each construct from four independent measurements (n = 4). Mean values (±SD) are reported. The molar ratio was calculated by comparing the myosin concentration to the concentration of the incorporated TMR.
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