Fig. 2: Reconstitution of luciferase via dual REVeRT AAVs in vivo and in human retinal organoids.

a Dual REVeRT AAVs expressing a split luciferase gene. Lower panel, Time scale for experiments in WT mice (b, c). b Luminescence obtained from WT mice co-injected with titer-matched dual REVeRT AAV9 vectors. Untreated mice injected with luciferin served as negative control. c Relative quantification of luciferase expression in different organs three weeks post-injection. n = 3 mice. RLU, relative light units. d Time scale for experiments in human retinal organoids transduced with dual REVeRT AAV9 vectors at differentiation day 215 (DD215). dpt, days post-transduction. e Luminescence obtained from transduced organoids. Regions of interest indicating the organoid position were used for quantification and are shown in red. A AAV vector expressing full-length luciferase served as positive control. f Quantification of the results shown in (e). n = 3 retinal organoids. Scatter plots show mean ± SEM. All source data in this figure are provided as a Source Data file.