Fig. 6: PRMT1 activity mediates the nucleolar shuttling of SMN. | Nature Communications

Fig. 6: PRMT1 activity mediates the nucleolar shuttling of SMN.

From: Nucleolar reorganization after cellular stress is orchestrated by SMN shuttling between nuclear compartments

Fig. 6: PRMT1 activity mediates the nucleolar shuttling of SMN.The alternative text for this image may have been generated using AI.

a Representative microscopy images of immunofluorescence (IF) assay showing the localization of SMN (red) and FBL (green) at different times Post UV-Irradiation (PUVI) in cells transfected with siMock or siPRMT1 pool. Nuclei and nucleoli are indicated by dashed and dotted lines respectively. Scale bar: 5 µm. b, c Quantification of cells number for localization of (b) SMN (in Cajal Bodies [CBs] or Gems, at the periphery of the nucleolus or mixed localization) and (c) FBL (inside the nucleolus, outside the nucleolus or mixed localization) at different times PUVI in cells transfected with siMock or siPRMT1 pool. At least 145 cells from one representative experiment were analyzed. d Representative microscopy images of IF assay showing the localization of SMN (red) and FBL (green) in MRC5 cells treated with DMSO, MS023 or Furamidine followed by 16 J/m² UV-C irradiation. Nuclei and nucleoli are indicated by dashed and dotted lines respectively. Scale bar: 5 µm. e Quantification of cells number for localization of SMN (in Cajal Bodies [CBs] or Gems, at the periphery of the nucleolus or mixed localization) at different times PUVI in cells treated with DMSO, MS023 or Furamidine. At least 60 cells from one representative experiment were analyzed. Source data of graphs are provided as a Source Data file.

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