Fig. 4: t-neopeptides activate T cells and induce cytotoxic responses. | Nature Communications

Fig. 4: t-neopeptides activate T cells and induce cytotoxic responses.

From: DNMT and HDAC inhibition induces immunogenic neoantigens from human endogenous retroviral element-derived transcripts

Fig. 4: t-neopeptides activate T cells and induce cytotoxic responses.The alternative text for this image may have been generated using AI.

a Representative example of flow cytometry-based characterization of PA*32-specific CD8+ T cells of a healthy volunteer (HV) after in vitro artificial antigen-presenting cell (aAPC)-priming with HLA-A*32-PA*32-monomer. b Frequencies of PA*32-specific CD8+ T cells compared to CD8+ T cells primed with an HLA-matched negative peptide in HVs (n = 3, frequency of T cells is indicated per well). All data points are shown, the band indicates the median, and the box indicates the first and third quartiles. c Representative example of IFN-γ and TNF production of PA*32-specific CD8+ T cells stimulated with PA*32 (upper panel) or an HLA-matched negative peptide (lower panel) after aAPC-priming. d Specific cell lysis by PA*32-specific CD8+ T cells of DAC + SB-treated NCI-H1299 cells at different effector-to-target cell ratios compared to PA*32-unspecific CD8+ T cells. Shown is the area of NCI-H1299 cells normalized to time point 0 h (t0) over time until 190 h. Results are shown as mean ± SEM for three independent technical replicates.

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