Fig. 1: Atp8b1IEC-KO mice (line #12) show a high infant mortality rate, growth retardation, and elongation of the SI.
From: Intestinal Atp8b1 dysfunction causes hepatic choline deficiency and steatohepatitis
a Atp8b1 mRNA levels in IEC and liver from male 4-week-old Atp8b1IEC-KO mice (line #12) (n = 12) and littermate Atp8b1flox/flox mice (n = 9). mRNA levels are expressed relative to those of 18 S rRNA. b Survival rate of male Atp8b1IEC-KO mice (line #12) and littermate Atp8b1flox/flox mice (n = 12–13 in each group). c–h Gross appearance of the whole body (c) and SI (d), BW (e), SIL to BW (f), SIW to BW (g), and LW to BW (h) in male newborn and 4-week-old Atp8b1IEC-KO mice (line #12) (n = 6 for newborn, n = 10 for 4wks) and littermate Atp8b1flox/flox mice (n = 5 for newborn, n = 10 for 4wks). All data are presented as mean ± SEM. P values were calculated by two-tailed, unpaired Welch’s t test and indicated in the figures if less than 0.05. BW body weight, IEC intestinal epithelial cells, LW liver weight, SIW small intestine weight, SIL small intestine length.
