Fig. 3: Atp8b1IEC-KO mice (line #12) progress to steatohepatitis.
From: Intestinal Atp8b1 dysfunction causes hepatic choline deficiency and steatohepatitis
Plasma and liver were collected from male Atp8b1IEC-KO mice (line #12) and littermate Atp8b1flox/flox mice at the newborn stage (n = 5) and at 4 weeks old (n = 6) and subjected to biochemical and histological analyses, respectively. a–d Plasma biochemistry; AST (a), ALT (b), T-bil (c), and D-bil (d). e H&E staining of liver section. f–i IHC staining of the liver section by antibodies against Plin2 (lipid droplets; f), MPO (neutrophils; g), F4/80 (macrophages; h), and GFAP (quiescent HSC; i). j–m Quantification of the area stained with Plin2 (j), MPO (k), F4/80 (l), and GFAP (m). Each symbol indicates values of 20 images/group from 5 mice/group. In (e–i), representative images are shown. Scale bars: 100 μm. In (a–d, j–m), all data are presented as mean ± SEM. P values were calculated by two-tailed, unpaired Welch’s t test and indicated in the figures if less than 0.05.
