Fig. 6: CSD eliminates steatohepatitis in Atp8b1IEC-KO mice (line #12).
From: Intestinal Atp8b1 dysfunction causes hepatic choline deficiency and steatohepatitis
Female mice mated to obtain Atp8b1IEC-KO mice (line #12) were fed a control diet (including 0.1% choline) or 1.0% CSD during lactation. The pups, Atp8b1IEC-KO mice (line #12) and littermate Atp8b1flox/flox mice, were analyzed immediately after weaning (4 weeks old). a Survival rate of Atp8b1IEC-KO mice (line #12) and littermate Atp8b1flox/flox mice (n = 14–16 in each group). b–e BW (b), SIW to BW (c), SIL to BW (d), and LW to BW (e) of Atp8b1IEC-KO mice (line #12) (n = 10 for control diet, n = 10 for CSD) and littermate Atp8b1flox/flox mice (n = 7 for control diet, n = 18 for CSD). f H&E staining of section from proximal and distal SI of Atp8b1IEC-KO mice (line #12) and littermate Atp8b1flox/flox mice. g Quantification of villus length. Each symbol indicates values of 40 villi from 5 mice in each group. h–k Plasma levels of AST (h), ALT (i), T-bil (j), and D-bil (k) of Atp8b1IEC-KO mice (line #12) [n = 12 for control diet, n = 8 for CSD (h–j); n = 6 per group (k)] and littermate Atp8b1flox/flox mice [n = 9 per group (h–j); n = 6 per group (k)]. l PC levels in liver from Atp8b1IEC-KO mice (line #12) and littermate Atp8b1flox/flox mice (n = 6 in each group). m–q HE (m) and IHC (n–q) staining of liver section from Atp8b1IEC-KO mice (line #12) and littermate Atp8b1flox/flox mice. Plin2 (lipid droplets; n), MPO (neutrophils; o), F4/80 (macrophages; p), and GFAP (quiescent HSC; q) were stained. r–u Quantification of the area stained with Plin2 (r), MPO (s), F4/80 (t), and GFAP (u). Each symbol indicates values of 20 images/group from 5 mice/group. In (f, m–q), representative images are shown. Scale bars: 100 μm. In (b–e, g–l, r–u), all data are presented as mean ± SEM. P values were calculated by Welch’s one-way ANOVA with a post hoc Dunnett’s T3 test for multiple comparisons and are indicated in the figures if less than 0.05. BW body weight, CSD choline supplemental diet, IEC intestinal epithelial cells, LW liver weight, SIW small intestine weight, SIL small intestine length.
