Fig. 8: Effect of Lcn2 deficiency on PA-regulated signaling pathways in BAT. | Nature Communications

Fig. 8: Effect of Lcn2 deficiency on PA-regulated signaling pathways in BAT.

From: Lipocalin 2 regulates mitochondrial phospholipidome remodeling, dynamics, and function in brown adipose tissue in male mice

Fig. 8

Protein expression levels of mTOR signaling components (a) and ERK (b) in BAT homogenate of WT and Lcn2 KO mice treated with Saline or CL316, 243 for 14 days. Time-course of ERK protein induction by CL316, 243 in differentiated brown adipocytes (c). Cell culture experiments were repeated twice independently. (d–g) quantification of western-blotting band intensity (n = 3 mice). Results are presented as mean ± SEM. Student’s t-test was performed to test differences between two independent groups. All tests were two-sided. The diagram depicts the disrupted processes of acyl-chain remodeling and metabolism of phospholipids and the altered signaling PA production and function in Lcn2 KO BAT (h). Blue arrows indicate changes in enzymes and lipids in the basal condition. Red arrows represent changes in the CL316, 243-treated condition. Source data are provided as a Source data file.

Back to article page