Fig. 1: sTREM2 attenuates tau hyperphosphorylation in vitro. | Nature Communications

Fig. 1: sTREM2 attenuates tau hyperphosphorylation in vitro.

From: Soluble TREM2 ameliorates tau phosphorylation and cognitive deficits through activating transgelin-2 in Alzheimer’s disease

Fig. 1

Western blots (a) and quantification (b) tau phosphorylation in HEK293-Tau cells treated with Fc, heat-inactivated Fc-sTREM2 (40 nM), or Fc-sTREM2 (40 nM) for 24 h (mean ± s.e.m.; one-way ANOVA, n = 5 independent experiments). Immunostaining (c) and quantification (d) of p-Tau S202 in HEK293-Tau cells treated with Fc (40 nM), heat-inactivated Fc-sTREM2 (40 nM), or Fc-sTREM2 (40 nM) for 24 h. Scale bar, 25 μm (mean ± s.e.m.; one-way ANOVA, n = 10 images from 5 independent experiments). p-Tau S202 immunostaining (e) and quantification (f) of HEK293-Tau cells treated with different concentrations of sTREM2. The control group was treated with Fc. Scale bars, 25 μm (mean ± s.e.m.; one-way ANOVA, n = 10 images from 5 independent experiments; compared with the control group). Western blots (g) and quantification (h) showing the phosphorylation of tau and GSK3β in HEK293-Tau cells treated with different concentrations of sTREM2. The control group (sTREM2 = 0 nM) was treated with Fc (mean ± s.e.m.; one-way ANOVA, n = 4 independent experiments; compared with the control group). p-Tau S202 and p-tau S396 immunostaining (i) and quantification (j) of primary neurons derived from tau P301S mice treated with different concentrations of sTREM2. Scale bar, 20 μm. The fluorescence intensity in the field was normalized to the control group (mean ± s.e.m.; one-way ANOVA, n = 10 images from 5 independent experiments; compared with the control group), *P < 0.05, **P < 0.01, ***P < 0.001.

Back to article page