Fig. 3: sTREM2 inhibits tau phosphorylation by activating TG2. | Nature Communications

Fig. 3: sTREM2 inhibits tau phosphorylation by activating TG2.

From: Soluble TREM2 ameliorates tau phosphorylation and cognitive deficits through activating transgelin-2 in Alzheimer’s disease

Fig. 3

a Western blots showing the phosphorylation of tau and GSK3β in HEK293-Tau cells treated with vehicle or TSG12 (25 μM). b Quantification of the immunoreactivity in (a) (mean ± s.e.m.; Two-tailed t-test, n = 4 independent experiments; compared with the control group). c p-Tau S202 immunostaining in HEK293-Tau cells treated with vehicle or TSG12 (mean ± s.e.m.; two-way ANOVA, n = 10 images from 5 independent experiments). Scale bars, 15 μm. d p-Tau S202 immunostaining in vehicle- or TSG12-treated (25 μM) primary neurons derived from tau P301S mice (mean ± s.e.m.; Two-tailed t-test, n = 10 images from 5 independent experiments). Scale bars, 20 μm. e HEK293-Tau cells were pretreated with sh-NC or sh-TG2, and then exposed to Fc-sTREM2 (20 nM) or control (Fc, 20 nM) for 24 h. Shown is the phosphorylation of tau and GSK3β. f Quantification of the immunoreactivity in (e) (mean ± s.e.m.; two-way ANOVA, n = 4 independent experiments). g p-Tau S202 immunostaining and quantification of HEK293-Tau cells pretreated with control shRNA (sh-NC) or sh-TG2 and then exposed to Fc-sTREM2 (20 nM) or control (Fc, 20 nM) for 24 h. Scale bars, 20 μm (mean ± s.e.m.; two-way ANOVA, n = 10 images from 5 independent experiments). h p-Tau S202 immunostaining and quantification of primary neurons derived from tau P301S mice pretreated with control shRNA (sh-NC) or sh-TG2 and then exposed to Fc-sTREM2 (20 nM) or control (Fc, 20 nM) for 24 h. Scale bars, 20 μm. (mean ± s.e.m.; two-way ANOVA, n = 10 images from 5 independent experiments). *P < 0.05, **P < 0.01, ***P < 0.001.

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