Fig. 6: Cross-linking affect the activity of Lss and LytM differently.
From: Staphylococcus aureus sacculus mediates activities of M23 hydrolases
a HADDOCK models oriented with respect to glycan strand. Ligand surface is coloured as in Fig. 1. Enzymes are shown in cartoon representation. b Schematic representation of LssCD and LytMCD interaction with double cross-linked muropeptide. Arrows indicate the shift of LytMCD towards the neighbouring cross-bridge. Distance between loop 1 and glycine of the cross-bridge was estimated in Pymol. PG residues were colour-coded as in Fig. 1. c Proposition of a model for the differentiated LssCD and LytMCD activity within the PG mesh. LssCD solubilises cross-linked PG, whereas LytMCD targets preferentially sites of sacculus that display decreased cross-linking.
