Fig. 1: Inactivation of MSH2 partially restores fertility in fancm zip4 Col × Ler hybrids.

a Representative images of wild-type, fancm zip4 and msh2 fancm zip4 Col × Ler hybrids. Scale bar, 2 cm. b Representative cleared siliques of wild-type, fancm zip4 and msh2 fancm zip4 Col × Ler hybrids. Scale bar, 1 cm. c, d Fertility assays in Col × Ler fancm zip4 and msh2 fancm zip4 as assessed via seed set (c) and silique length (d). The centre line of a boxplot indicates the mean; the upper and lower bounds indicate the 75th and 25th percentiles, respectively. Each dot represents a measurement from five siliques of one plant. The numbers of individuals are indicated above the boxplots. The two-sided P values were estimated by Welch’s t test. e DAPI-stained metaphase I chromosome spreads from Col × Ler male meiocytes in wild type, fancm zip4 and msh2 fancm zip4. Scale bars, 5 μm. f Proportions of rings, rods and univalents per Col × Ler male meiocyte in wild type, fancm zip4 and msh2 fancm zip4. Three individual plants per genotype were scored. The total number of cells sampled for each genotype is indicated above the bars. g 420 crossover frequency (cM) in Col × Ler hybrids of fancm, msh2, msh2 fancm, fancm zip4 and msh2 fancm zip4. The two-sided P values were estimated by Welch’s t test. The centre line of a boxplot indicates the mean; the upper and lower bounds indicate the 75th and 25th percentiles, respectively. Each dot represents a measurement from one individual. The numbers of individuals are indicated above the boxplots. h Genetic diagram illustrating the seed scoring approach with a single chromosome pair shown for simplicity. Fluorescent reporters in FTL are indicated as green and red triangles. FTL in the Col background (grey) is crossed to Ler (blue) to generate F₁ hybrids. Following meiosis the proportion of parental:crossover gametes from F₁ is analysed to measure genetic distance (cM) between the fluorescent protein-encoding transgenes. Top panel shows the representative photographs of seed fluorescence segregation in 420 interval. Scale bars, 3 mm. Source data are provided as a Source Data file.