Fig. 3: Mobility of rapidly bound ORCJF646 on 10.4 kbp DNA containing an ARS1 (or mutated) origin (chromatinized or not). | Nature Communications

Fig. 3: Mobility of rapidly bound ORCJF646 on 10.4 kbp DNA containing an ARS1 (or mutated) origin (chromatinized or not).

From: A chromatinized origin reduces the mobility of ORC and MCM through interactions and spatial constraint

Fig. 3: Mobility of rapidly bound ORCJF646 on 10.4 kbp DNA containing an ARS1 (or mutated) origin (chromatinized or not).The alternative text for this image may have been generated using AI.

Dataset acquired as described in Fig. 2a. a Traces collected on DNA containing a chromatinized origin illustrating motion of ORCJF646: (i) initially localized within the bin containing the chromatinized origin or (ii) initially localized elsewhere. b Histograms of the diffusion constants of ORCJF646 on DNA molecules containing (i) a non-chromatinized origin, (ii) a chromatinized origin, (iii) a non-chromatinized mutated origin, and (iv) a chromatinized mutated origin. Only foci containing 1 or 2 ORCJF646 are included in the analysis (Nfoci). Data are presented as mean values ± one-sigma Wilson confidence intervals. The observed bimodal distribution is fitted to a double log-normal function (solid black line) which identifies a slow ORC population (55% of the distribution with 0.0049±  0.0028 kbp2/s, mean ± SEM) and a fast ORC population (0.152 ±  0.023 kbp2/s, mean ± SEM). The dashed line indicates the average of the means of the two normal distributions used to fit log10(D); this average is used as a threshold (D*). The means of these subpopulations are imposed in the fits to the data in (ii–iv). Created with BioRender.com. c Examination of the slow ORC population. (i) Fitted proportion of the slow ORC population for the datasets in (b-i)-(b-iv), as indicated by the corresponding color bar (ARS1: non-mutated origin; Mut ori: mutated origin; nuc: chromatinized). (ii) The population of ORC initially localized within the bin containing the (chromatinized or not) origin was extracted from the dataset; the fraction of this population that is slow (D < D*) is shown here. (iii) The population of ORC initially localized outside the bin containing the (chromatinized or not) origin was extracted from the dataset; the fraction of this population that is slow (D < D*) is shown here. Nfoci-ARS1 = 69; Nfoci-ARS1+nuc = 35; Nfoci-Mut ori = 40; Nfoci-Mut ori+nuc = 27. Data are presented as mean values ± one-sigma Wilson confidence intervals. Statistical significance is determined by a two-sided binomial test: n. s. not significant, * p < 0.05, ** p < 0.005, **** p < 0.00005, ***** p < 0.000005 ****** p < 0.0000005. Source data are provided as a Source Data file.

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